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采用ＭＴＴ法测定人胎儿垂体培养上清（ＦＰＳ）刺激胎脾淋巴细胞增殖反应。实验中观察到：（１）ＦＰＳ能刺激胎脾淋巴细胞增殖，其刺激能力随胎龄增大而增强；（２）胎儿垂体能在体外培养１ｍｏ内持续分泌对淋巴细胞增殖的有效刺激成分；（３）ＦＰＳ能延长胎脾淋巴细胞体外培养存活时间；（４）ＰＨＡ对ＦＰＳ刺激胎脾淋巴细胞增殖无影响。实验结果显示ＦＰＳ对人胎脾淋巴细胞在体外有刺激增殖作用。
The proliferation of fetal spleen lymphocytes stimulated by human fetal pituitary culture supernatant (FPS) was measured by MTT assay. Experiments were observed: (1) FPS can stimulate the proliferation of fetal spleen lymphocytes, and its stimulation ability increases with gestational age increased; (2) fetal pituitary in vitro 1mo sustained secretion of lymphocyte proliferation effective stimuli; (3) FPS can prolong the survival time of fetal spleen lymphocytes cultured in vitro; (4) PHA had no effect on the proliferation of fetal spleen lymphocytes stimulated by FPS. The experimental results show that FPS stimulates proliferation of human fetal spleen lymphocytes in vitro.