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采用MTT法测定人胎儿垂体培养上清(FPS)刺激胎脾淋巴细胞增殖反应。实验中观察到:(1)FPS能刺激胎脾淋巴细胞增殖,其刺激能力随胎龄增大而增强;(2)胎儿垂体能在体外培养1mo内持续分泌对淋巴细胞增殖的有效刺激成分;(3)FPS能延长胎脾淋巴细胞体外培养存活时间;(4)PHA对FPS刺激胎脾淋巴细胞增殖无影响。实验结果显示FPS对人胎脾淋巴细胞在体外有刺激增殖作用。
The proliferation of fetal spleen lymphocytes stimulated by human fetal pituitary culture supernatant (FPS) was measured by MTT assay. Experiments were observed: (1) FPS can stimulate the proliferation of fetal spleen lymphocytes, and its stimulation ability increases with gestational age increased; (2) fetal pituitary in vitro 1mo sustained secretion of lymphocyte proliferation effective stimuli; (3) FPS can prolong the survival time of fetal spleen lymphocytes cultured in vitro; (4) PHA had no effect on the proliferation of fetal spleen lymphocytes stimulated by FPS. The experimental results show that FPS stimulates proliferation of human fetal spleen lymphocytes in vitro.