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目的:探讨危重病人预警蛋白质(lostgoodwilltarget,LGT)多肽谱指纹的峰型和丰度对判断肿瘤患者病情发展及预后的意义。方法:对接受SELD(ISurface enhanced laser desorption/ionization-time of flight-masss pectrometer)技术检测的178名患者生存期与死亡时间进行调查,并根据SELDI检测所获得的指纹图中,以LGT指纹存在与否,分成无峰组、单峰组、双峰组、三峰和多峰组。采用多因素方差分析和COX风险回归模型分析的方法,比较四组中哪一种状态下死亡风险最大。结果:恶性肿瘤患者经SELDI技术检测所获取的指纹图中,如果LGT呈阴性表达,死亡风险低于单、双峰组,远低于三峰和多峰组;LGT呈三峰和多峰表达,其死亡风险远高于单、双峰组,差异有统计学意义(P<0.05),死亡率极高。结论:采用SELDI技术检测肿瘤患者的血清,LGT出现三峰和多峰表达,提示肿瘤患者病情即将恶化,该指纹标识为LGT阳性指标;单、双峰者为疑似指标;LGT指纹阴性表达,肿瘤患者近期预后良好,死亡风险极小。
Objective: To investigate the significance of peak shape and abundance of fingerprints of patients with critically ill patients’ LGG polypeptide in judging the progression and prognosis of cancer patients. METHODS: The survival and death of 178 patients undergoing SELD (ISurface enhanced laser desorption / ionization-time of flight-mass pectrometer) technique were investigated. According to the fingerprint obtained by SELDI, No, divided into non-peak group, unimodal group, bimodal group, tri-peak and multi-peak group. Multivariate ANOVA and COX risk regression model analysis were used to compare which of the four groups had the highest risk of death. Results: In the fingerprints obtained from patients with malignant tumors detected by SELDI technique, if the LGT was negatively expressed, the risk of death was lower than that of the single and double peak groups and much lower than that of the triple and multi-peak groups; The risk of death was much higher than that in single and bimodal groups, the difference was statistically significant (P <0.05), and the mortality rate was extremely high. Conclusion: SELDI technique was used to detect the serum of patients with tumors. The expression of three peaks and multiple peaks of LGT showed that the tumor patients were about to deteriorate. The fingerprint was LGT positive index; single and double peak was the suspected index; LGT fingerprint negative expression, The recent prognosis is good, with minimal risk of death.