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为了研究LNCaP前列腺癌疾病,试验采用在SCID小鼠前列腺内接种LNCaP前列腺癌细胞建立原位接种模型并探讨其应用价值,用微量注射器在SCID小鼠的前列腺左右背外侧叶包膜下接种小鼠前列腺癌LNCaP细胞1×106个建立原位移植瘤模型,以皮下移植瘤模型作为对照比较两者的生存期、肿瘤转移发生情况,并在电镜下观察LNCaP前列腺肿瘤细胞和小鼠正常前列腺组织细胞的特征。结果表明:原位模型组小鼠平均生存时间为(21.0±2.1)d,较对照组(35.0±4.5)d明显缩短,差异极显著(P<0.01);原位模型组小鼠全部发生肿瘤转移,其中盆腔淋巴结和肺脏的转移发生率分别为100%(15/15)、60%(9/15);电镜观察发现,SCID小鼠的正常前列腺细胞与癌变后的细胞体积及细胞器均有很大差异。
In order to study the LNCaP prostate cancer disease, inoculation models of LNCaP prostate cancer cells inoculated in the prostate of SCID mice were established and their application value was discussed. Mice were inoculated subcutaneously in the left and right posterior leaves of SCID mice with a micro syringe Prostate cancer LNCaP cells 1 × 106 in situ xenograft model was established, subcutaneous xenograft model was used as a control to compare the survival and tumor metastasis, and observed under electron microscope LNCaP prostate tumor cells and mouse normal prostate tissue cells Characteristics. The results showed that the average survival time of mice in situ model group was (21.0 ± 2.1) d, which was significantly shorter than that in control group (35.0 ± 4.5) d, the difference was significant (P <0.01) The incidence of pelvic lymph nodes and lung metastases were 100% (15/15) and 60% (9/15), respectively. Electron microscopy showed that both the normal prostate cells and the cancerous cells and organelles of SCID mice had Huge difference.