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目的:探讨p75NTR基因对舌鳞癌Tca8113细胞凋亡的影响。方法:流式细胞技术分选Tca8113细胞系中p75NTR阳性细胞及阴性细胞,对阳性细胞用脂质体瞬时转染荧光标记的p75NTR siRAN,实时定量RT-PCR检测p75NTR基因,western blot检测p75NTR蛋白表达;流式细胞仪Annexin V/PI双染色标记法进行凋亡检侧;MTT检测细胞增殖的影响。结果:p75NTR表达阳性的Tca8113细胞经p75NTR siRNA转染后p75TNR的表达被抑制;细胞凋亡率明显增加(P<0.05),细胞增殖率明显降低。结论:p75NTR在Tca8113细胞凋亡中具有调控作用。
Objective: To investigate the effect of p75NTR gene on the apoptosis of tongue squamous cell carcinoma Tca8113 cells. Methods: p75NTR positive cells and negative cells were sorted by flow cytometry. The positive cells were transiently transfected with p75NTR siRAN by lipofectamine. The p75NTR gene was detected by real-time quantitative RT-PCR. The expression of p75NTR protein was detected by western blot. ; Flow cytometry Annexin V / PI double staining for apoptosis detection; MTT detection of cell proliferation. Results: The expression of p75TNR in p75NTR siRNA transfected Tca8113 cells with p75NTR expression was inhibited. The apoptosis rate was significantly increased (P <0.05), and the cell proliferation rate was significantly decreased. Conclusion: p75NTR plays a regulatory role in Tca8113 cell apoptosis.