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目的侵袭与转移是影响膀胱癌患者疗效及导致死亡的主要因素,其具体机制尚不明确;研究表明miR-451与多种肿瘤的发生、发展密切相关。本研究分析miR-451在膀胱癌中的表达及其临床意义,观察miR-451表达改变对上皮型钙黏附蛋白(E-cadherin)和间质型骨架蛋白(Vimentin)表达的影响。方法收集2012-01-01-2015-12-31江西省人民医院收治的47例膀胱癌患者的癌组织及10例正常膀胱组织标本,运用实时荧光定量PCR(quantitative Real-time PCR,qPCR)检测miR-451的表达及运用免疫组织化学技术检测E-cadherin和Vimentin的表达,分析miR-451表达与膀胱癌临床病理因素,以及E-cadherin和Vimentin表达的关系。利用脂质体将miR-451拟似物(miR-451mimics)转染入膀胱癌5637细胞,采用蛋白质印迹法检测E-cadherin和Vimentin的表达。结果 miR-451在膀胱癌组织中的表达为0.14±0.017,明显低于正常膀胱组织(1.0),其表达和膀胱癌临床分期及病理分级密切相关,P<0.01;E-cadherin在膀胱癌组织较正常组织中低表达,分别为36.17%和84.50%;Vimentin在膀胱癌组织及正常膀胱黏膜层中表达不明显。癌组织中miR-451表达与E-cadherin表达正相关,r=0.67,P=0.023;miR-451过表达后,E-cadherin蛋白表达上调(P<0.001),Vimentin蛋白表达无明显改变,P>0.05。结论MiR-451是膀胱癌潜在的调控子,可能是通过调控E-cadherin蛋白表达进而影响膀胱癌发生及进展。
The purpose of invasion and metastasis is to affect the efficacy of bladder cancer patients and lead to death of the main factors, the specific mechanism is not clear; studies have shown that miR-451 and a variety of tumor occurrence and development are closely related. This study analyzed the expression of miR-451 in bladder cancer and its clinical significance, and observed the effect of miR-451 expression on the expression of E-cadherin and Vimentin. Methods Forty-seven cases of bladder cancer and 10 cases of normal bladder tissue from Jiangxi Provincial People’s Hospital were collected and analyzed by quantitative real-time PCR (qPCR) The expression of miR-451 and the expression of E-cadherin and Vimentin were detected by immunohistochemistry. The relationship between the expression of miR-451 and the clinicopathological parameters of bladder cancer, E-cadherin and Vimentin was analyzed. The miR-451 mimics were transfected into bladder cancer 5637 cells by liposomes, and the expressions of E-cadherin and Vimentin were detected by Western blotting. Results The expression of miR-451 in bladder cancer was 0.14 ± 0.017, which was significantly lower than that in normal bladder tissue (1.0). The expression of miR-451 was closely related to the clinical stage and pathological grade of bladder cancer (P <0.01) The expression of Vimentin was lower in bladder cancer tissues and normal bladder mucosa than in normal tissues (36.17% vs 84.50%, respectively). The expression of miR-451 was positively correlated with the expression of E-cadherin (r = 0.67, P = 0.023). The expression of E-cadherin was up-regulated after miR-451 overexpression (P <0.001) > 0.05. Conclusion MiR-451 is a potential regulator of bladder cancer, which may affect the occurrence and progression of bladder cancer by regulating the expression of E-cadherin protein.