引言传统的缺磷诊断方法(如叶片总磷量分析、可见症状的鉴定)存在一定局限性,因而对以酶标示的缺磷诊断的研究颇感兴趣.各种作物(包括小麦)的叶片缺磷能引起一至数种磷酸酶活性的提高.这些酶主要催化各种磷酸单酯的水解反应.磷酸酶易被提取,也较稳定,可以用硝基苯基磷酸盐为基质,通过简单的比色法加以迅速测定.Besford最近指出,叶片磷酸酶活性的测定有可能作为缺磷的一种准确的生物化学标志. Introduction Traditional methods for the determination of phosphorus deficiency (such as total phosphorus analysis of leaves and the identification of visible symptoms) have some limitations and are therefore of interest for the diagnosis of phosphorus-deficient phosphorus. The leaves of various crops (including wheat) lack Phosphorus can cause an increase in the activity of one or several phosphatases, which catalyze the hydrolysis of various monoesters of phosphoric acid, which are easily extracted and are also more stable, can be treated with nitrophenyl phosphate as a substrate, The colorimetric method was also used to quickly determine the extent of the change in leaf phosphatase activity, as Besford recently pointed out may be an accurate biomarker for phosphorus deficiency.