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目的 为心肌缺血 /再灌注损伤所致心性猝死的法医学诊断提供形态学依据 ,方法运用免疫组织化学方法 ,研究Bax蛋白在心肌组织中不同区域的表达。 结果 对照组正常心肌组织无Bax蛋白表达。Bax蛋白再灌注 0 .5h后即可在缺血区域表达 ,且表达主要在心肌内层。再灌注 1h后可观察到Bax蛋白不仅表达于心肌内层且已经扩展至心肌的全层。再灌注 2h发现心肌外层表达明显强于心肌内层 ,随着再灌注时间的延长可以发现Bax蛋白的表达逐渐减弱。 结论 在急性心肌缺血再灌注中 ,Bax基因对心肌细胞凋亡的发生有着重要的作用 ,且可以作为心肌缺血再灌注的早期诊断提供一项辅助诊断依据。
Objective To provide a morphological basis for forensic diagnosis of sudden cardiac death caused by myocardial ischemia / reperfusion injury. Methods Immunohistochemistry was used to study the expression of Bax in different regions of myocardium. Results There was no Bax protein expression in normal myocardium in control group. Bax protein can be expressed in the ischemic area at 0.5 h after reperfusion, and its expression is mainly in the myocardium. One hour after reperfusion, Bax protein was observed not only in the myocardium but also in the myocardium. After 2h of reperfusion, the expression of myocardial extracellular layer was obviously stronger than that of the myocardium. With the reperfusion time prolonging, the expression of Bax protein gradually decreased. Conclusions Bax gene plays an important role in cardiomyocyte apoptosis in acute myocardial ischemia-reperfusion injury, and may provide an auxiliary diagnosis basis for early diagnosis of myocardial ischemia-reperfusion injury.