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为探讨测定羊水中葡萄糖含量对诊断宫内感染的意义,选择孕34周前早产及/或早破水的39例孕妇,除外糖尿病、7天内应用过抗菌素及入院时宫口开大>4cm 者。所有病例均在 B 超下用无菌操作,以20号腰穿针作羊膜腔穿刺抽取羊水进行涂片革兰氏染色检查、培养及药敏试验,同时作人支原体和脲支原体培养,用葡萄糖氧化酶法测定羊水中葡萄糖含量,同时取孕妇静脉血测定血糖。宫内感染诊断依据:羊水培养阳性、胎盘和/或脐带中有多核白细胞、羊水涂片革兰氏染色见到细菌。宫内感染和羊水中葡萄糖含量的关系用 t 检验,P<0. 05有意义。
In order to explore the significance of determination of glucose in amniotic fluid for the diagnosis of intrauterine infection, 39 pregnant women with premature and / or premature rupture of water 34 weeks before pregnancy were selected, with the exception of diabetes. Antibiotics were used within 7 days and those with cervix greater than 4 cm at admission were selected. All cases were treated with aseptic technique under ultrasound B, amniocentesis on the 20th of lumbar puncture for amniotic fluid extraction, smear Gram stain examination, culture and drug sensitivity test, at the same time as human mycoplasma and ureaplasma urealyticum cultured with glucose Oxidation method for the determination of amniotic fluid glucose content, taking the determination of blood glucose in pregnant women. Diagnosis of intrauterine infection diagnosis: positive amniotic fluid culture, placental and / or umbilical cord in the multicellular leukocytes, amniotic fluid smear Gram stain see bacteria. The relationship between intrauterine infection and amniotic fluid glucose content using t test, P <0 05 meaningful.