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中国医科院肿瘤研究所 在癌生物学最近的研究工作中,癌基因在肿瘤发生发展中的作用正日益受到重视。近年来杂交技术的发展为研究癌基因在细胞中的作用及肿瘤的发生机制提供了强有力的工具。这些分子生物学的分析技术检测的是整体细胞群体,因此,资料反映了群体细胞或组织的平均变化情况。细胞原位杂交则是在单细胞水平上检测细胞的基因表达变化。我们用RNA-RNA原位杂交的方法检测了原发性肝癌组织C-myc和H-ras-1的基因表达变化。
Cancer Institute of Chinese Academy of Medical Sciences In the latest research work in cancer biology, the role of oncogenes in the development of tumors is increasingly receiving attention. In recent years, the development of hybridization technology has provided a powerful tool for studying the role of oncogene in cells and the mechanism of tumorigenesis. These molecular biology analysis techniques detect the entire cell population. Therefore, the data reflect the average changes in population cells or tissues. In situ hybridization of cells is the detection of cell gene expression changes at the single cell level. We used RNA-RNA in situ hybridization to detect changes in gene expression of C-myc and H-ras-1 in primary hepatocellular carcinoma.