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本文用PTIO(2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide,NO清除剂)、过氧化氢酶(CAT,H_2O_2清除剂)和ニ亚苯基氯化碘盐(DPI,质膜NADPH氧化酶抑制剂)处理小麦(Triticum aestivum)新品种‘陇春27’,探讨了内源NO与活性氧(ROS)的产生及其对幼苗生长、抗氧化反应以及渗透性调节的影响。结果显示:CAT只抑制小麦根的生长,而PTIO或DPI处理均抑制小麦幼苗根和茎的生长。PTIO处理下幼苗根叶中NO、叶中总ROS含量均減少,而根中总ROS和胞间ROS含量増加;CAT的加入导致叶中NO、总H_2O_2及根质外体H_2O_2含量均降低,而叶中羟自由基(·OH)含量及根NO、总超氧阴离子(O_·~-)和质外体O_2·~-含量均升高;相比,DPI处理下幼苗叶中NO量减少而根中的量增加,同时,DPI诱导幼苗叶中总H_2O_2及根质外体ROS含量均減少,而使根中总H_2O_2及根叶中总O_2·~-和·OH含量显著增加。PTIO、CAT或DPI处理下小麦幼苗根叶中超氧化物歧化酶(SOD)、CAT和过氧化物酶(POD)的活性均呈现不同的变化趋势。此外,PTIO处理使幼苗可溶性糖和可溶性蛋白含量增加,CAT不影响渗透性调节物的含量,但DPI处理下脯氨酸、可溶性糖和可溶性蛋白含量均增加,显著提高渗透调节能力。PTIO或DPI均诱导了幼苗丙二醛(MDA)含量的增加,且DPI处理下的増幅最大。研究表明小麦幼苗根叶中内源性NO和胞间ROS的产生相互影响,特别是NO和胞间ROS影响ROS和抗氧化酶的代谢,导致小麦根和叶表现出不同的抗氧化反应。
In this paper, PTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide, NO scavenger), catalase (CAT, H 2 O 2 scavenger) (DPI, a plasma membrane NADPH oxidase inhibitor) was used to treat ’Longchun 27’, a new wheat cultivar Triticum aestivum. The effects of endogenous NO and reactive oxygen species (ROS) on seedling growth, antioxidant activity and permeability Effect of regulation. The results showed that: CAT only inhibited the growth of wheat roots, while PTIO or DPI inhibited the growth of wheat seedling roots and stems. Under PTIO treatment, the content of NO and leaf total ROS in seedling leaves decreased, but the content of total ROS and interstitial ROS in root increased, while the content of NO, total H_2O_2 and H_2O_2 in root increased. The content of hydroxyl radical (· OH) and the contents of NO, total superoxide anion (O_ · -) and the content of protoplast O_2 · - were increased in leaves. Compared with the control, At the same time, the contents of total H 2 O 2 and extracellular ROS in seedling leaves decreased with DPI, and the content of total H 2 O 2 and total O 2 ·· - · OH in root and root increased significantly. The activities of superoxide dismutase (SOD), peroxidase (CAT) and peroxidase (POD) in roots and leaves of wheat seedlings all showed different trends under PTIO, CAT or DPI treatments. In addition, the treatment of PTIO increased the content of soluble sugar and soluble protein in seedlings, and CAT did not affect the content of osmotica. However, the content of proline, soluble sugar and soluble protein in DPI increased significantly and osmotic adjustment ability was significantly improved. PTIO or DPI all induced the increase of malondialdehyde (MDA) content in seedlings, and the largest increase was observed under DPI treatment. The results showed that the production of endogenous NO and intercellular ROS in root and leaf of wheat seedlings interacted each other. In particular, NO and intercellular ROS affected the metabolism of ROS and antioxidant enzymes, resulting in different antioxidant and antioxidant responses in wheat roots and leaves.