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建立了用凝胶渗透色谱-高效液相色谱法测定植物油中黄曲霉毒素B1、B2、G1、G2的方法。该方法以乙酸乙酯-环己烷(1:1,v/v)提取样品,凝胶渗透色谱净化,以Extend C18柱(4.6mm×250 mm,5μm)分离,流动相为甲醇和10 mmol/L乙酸铵水溶液(45+55,v/v),流速1.0 mL/min,激发波长360 nm,发射波长440 nm,柱温25℃,进样量50μL。黄曲霉毒素B1、B2、G1、G2的检出限分别为2.0、1.0、2.0、1.0μg/kg,4种毒素在2.0~30、1.0~10、2.0~30、1.0~10μg/kg范围内分别为0.9999、0.9997、0.998、0.9996;加标回收率82.6%~98.4%,相对标准偏差为4.97%~9.79%。
A method for the determination of aflatoxins B1, B2, G1, G2 in vegetable oil by gel permeation chromatography - high performance liquid chromatography was established. The sample was extracted with ethyl acetate-cyclohexane (1: 1, v / v) and purified by gel permeation chromatography. The residue was separated on an Extend C18 column (4.6 mm × 250 mm, 5 μm) using methanol and 10 mmol / L aqueous ammonium acetate solution (45 + 55, v / v) at a flow rate of 1.0 mL / min with an excitation wavelength of 360 nm and an emission wavelength of 440 nm at a column temperature of 25 ° C. The detection limits of aflatoxins B1, B2, G1 and G2 were 2.0,1.0,2.0,1.0μg / kg, and the four toxins were in the range of 2.0-30, 1.0-10, 2.0-30, 1.0-10μg / kg Respectively, 0.9999,0.9997,0.998,0.9996; spike recovery 82.6% ~ 98.4%, the relative standard deviation of 4.97% ~ 9.79%.