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目的研究医院环境分离凝固酶阴性葡萄球菌分布及其对苯扎溴铵消毒液抗力的变化情况。方法采用细菌检测和肉汤稀释法,对医院环境标本进行了细菌分离鉴定并测定分离的凝固酶阴性葡萄球菌对苯扎溴铵的最小抑菌浓度(MIC)。结果从841份医院环境表面和医护人员手标本中共分离出63株凝固酶阴性葡萄球菌,分离率为7.49%;从中检出12株携带qacA基因的凝固酶阴性葡萄球菌。苯扎溴铵对携带qacA基因的12株凝固酶阴性葡萄球菌MIC值范围为0.7 mg/L～2.8 mg/L,其中11株的MIC值均高于金黄色葡萄球菌标准株。结论携带qacA基因的凝固酶阴性葡萄球菌对苯扎溴铵的抗力普遍高于金黄色葡萄球菌消毒试验标准菌株。
Objective To study the distribution of coagulase-negative staphylococci in the hospital environment and the changes of resistance to benzalkonium bromide disinfectant. Methods Bacteria detection and broth dilution method were used to isolate and identify the bacterial isolates from hospital environmental samples and determine the minimum inhibitory concentration (MIC) of isolates of coagulase-negative staphylococci to benzalkonium bromide. Results A total of 63 strains of coagulase-negative staphylococci were isolated from 841 hospital environment surfaces and hand samples of medical staff. The isolation rate was 7.49%. 12 strains of coagulase-negative staphylococci carrying qacA gene were detected. The MIC value of benzalkonium bromide against 12 coagulase-negative staphylococci carrying qacA gene ranged from 0.7 mg / L to 2.8 mg / L, of which 11 were higher than that of Staphylococcus aureus. Conclusion The resistance of clot-bearing Staphylococcus aureus to benzalkonium bromide carrying qacA gene is generally higher than that of the standard strain of Staphylococcus aureus.