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目的：研究同一血清样品中喷昔洛韦和拉米夫定的高效液相色谱测定法。方法：在ＯＤＳ柱上，以０．０２ｍｏｌ／Ｌ磷酸盐缓冲液（ｐＨ ７．０）－甲醇－乙腈（９１：９：０．１）为流动相，在２７４ｎｍ紫外波长处检测；血清样品采用１０％高氯酸和２０％三氯乙酸直接沉淀蛋白，三氯甲烷抽提血清杂质成分，离心后取上清进样，观察分析从一血清样品中同时测定喷昔洛韦和拉米夫定的色谱分离效果。结果：在本选定条件下，喷昔洛韦和拉米夫定及其与血清杂质峰分离良好，两者的线性范围均为０．０５０～４．０ｍｇ／Ｌ（喷昔洛韦ｒ＝０．９９９３，拉米夫定ｒ＝０．９９９７），回收率均大于９５％（ｎ＝５），日内、日间精密度（ＲＳＤ）小于８％（ｎ＝５）。结论：本方法定量准确，简便易行，结果适用于同时服用泛昔洛韦和拉米夫定的ＨＢＶ感染者血清样本定量分析和临床相关研究。
OBJECTIVE: To study high performance liquid chromatographic determination of penciclovir and lamivudine in the same serum sample. Methods: ODS column was used to detect the UV wavelength at 274nm with 0.02mol / L phosphate buffer (pH 7.0) - methanol - acetonitrile (91: 9: 0.1) 10% perchloric acid and 20% trichloroacetic acid directly precipitated protein, chloroform extract of serum impurities, after centrifugation supernatant injection, observation and analysis of a serum sample from simultaneous determination of penciclovir and lamivudine The chromatographic separation effect. Results: Under the selected conditions, the concentrations of penciclovir and lamivudine were well separated from the serum impurity peaks, and the linear range of both was 0.050 ~ 4.0 mg / L (penciclovir r = 0.9993, and lamivudine r = 0.9997). The recoveries were both higher than 95% (n = 5). The intra-day and inter-day RSD was less than 8% (n = 5). Conclusion: The method is accurate and simple, and the results are suitable for the quantitative analysis and clinical research of serum samples from patients with HBV infected with both famciclovir and lamivudine.