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目的:探讨卵巢正常反应型妇女中,年龄与颗粒细胞卵泡刺激素受体(follicle-stimulating hormone receptor,FSHR)表达的关系。方法:根据年龄将90例卵巢正常反应型妇女分为A组:>37岁(30例),B组:35～37岁(30例),C组:<35岁(30例)。采用蛋白印迹法测定颗粒细胞FSHR蛋白的水平。电化学发光法测定卵泡液(follicularfluid,FF)中的FSH浓度。比较各组成熟卵泡数、重组FSH(rFSH)使用剂量的差异。结果:3组之间rFSH使用剂量(A组:3494.0±1086.9IU,B组:3000.8±902.9IU,C组:2510.0±726.8IU)、卵泡液FSH浓度(A组:8.6±0.6pmol/L,B组:8.2±0.7pmol/L,C组:7.2±0.6pmol/L)和成熟卵泡数(A组:6.9±1.9,B组:7.9±1.9,C组:9.1±1.6)有显著差异(P均<0.01)。随着年龄增长,FSHR蛋白相对表达量显著降低(A组:20.28±0.08,B组:0.32±0.08,C组:0.36±0.06),P<0.01。结论:年龄与颗粒细胞FSHR蛋白表达有关。临床上对大龄妇女采取增加rFSH剂量的方法并不能增加成熟卵泡数的原因可能与FSHR表达下降有关。
Objective: To investigate the relationship between age and the expression of follicle-stimulating hormone receptor (FSHR) in normal ovarian response women. Methods: Ninety ovarian reactive normal women were divided into three groups according to their age: group A (n = 37), group B (n = 35), group C (n = 30) and group C The level of FSHR protein in granulosa cells was determined by Western blot. Determination of FSH in Follicular Fluid (FF) by Electrochemiluminescence. The difference of dosage between the number of mature follicles and recombinant FSH (rFSH) in each group was compared. Results: The dosage of FSH in group A (3494.0 ± 1086.9IU in group A, 3000.8 ± 902.9IU in group B, 2510.0 ± 726.8IU in group C) and FSH in follicular fluid (group A: 8.6 ± 0.6 pmol / L, B group: 8.2 ± 0.7 pmol / L, C group: 7.2 ± 0.6 pmol / L) and mature follicles (A group: 6.9 ± 1.9, B group: 7.9 ± 1.9, C group: 9.1 ± 1.6) P <0.01). With age, the relative expression of FSHR protein was significantly decreased (A group: 20.28 ± 0.08, B group: 0.32 ± 0.08, C group: 0.36 ± 0.06, P <0.01). Conclusion: Age is related to the expression of FSHR protein in granulosa cells. Clinically, older women may not be able to increase the number of mature follicles by increasing the dosage of rFSH, which may be related to the decreased expression of FSHR.