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目的:观察大鼠心肌缺血或心肌缺血再灌注损伤(RMMI)时,心肌组织中N-Mcy下游调节基因(NDRG2)表达的变化。方法:将60只健康成年SD大鼠随机分为对照组、单纯缺血组和缺血/再灌注(I/R)组,每组5~6只。单纯缺血组:套扎冠脉,选取缺血3、6、12、24 h 4个时间点。再灌注组分别选择再灌注后6、12、24和48 h后获取心脏。结果:单纯缺血组各时间点NDRG2的表达与对照组相比无显著差异,NDRG2 mRNA和其蛋白表达的变化一致。再灌注组与对照组比较,随着时间的延长,NDRG2 mRNA的表达逐渐降低,在缺血12 h达到最低(P<0.01),NDRG2蛋白的表达在缺血24 h达到最低(P<0.01)。结论:单纯心肌缺血不引起NDRG2基因及其蛋白表达水平的变化,但再灌注损伤可下调心肌细胞中NDRG2的表达。
OBJECTIVE: To observe the expression of N-methylcystatin (NDRG2) in myocardial tissue during myocardial ischemia or myocardial ischemia-reperfusion injury (RMMI) in rats. Methods: Sixty healthy adult SD rats were randomly divided into control group, ischemia group and ischemia / reperfusion (I / R) group, with 5 to 6 rats in each group. Pure ischemia group: ligation of coronary artery, select the ischemic 3,6,12,24 h 4 time points. Reperfusion group were selected after 6, 12, 24 and 48 h after reperfusion heart. Results: Compared with the control group, the expression of NDRG2 in N group was not significantly different at each time point. The expression of NDRG2 mRNA and its protein were the same. Compared with the control group, the expression of NDRG2 mRNA gradually decreased after reperfusion, and reached the lowest level at 12 h (P <0.01). The expression of NDRG2 protein reached the lowest level at 24 h after ischemia (P <0.01) . Conclusion: Myocardial ischemia can not induce the change of NDRG2 gene and protein expression, but reperfusion injury can down-regulate the expression of NDRG2 in cardiomyocytes.