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为从形态和超微结构上观察干燥保存角膜内皮细胞是否具有活性，应用０．２％茜素红和０．２５％台盼兰对干燥保存不同时间的兔、猴和人角膜内皮细胞分别进行活性染色，并与正常新鲜者作对照。同时，在电镜下对干燥保存角膜及其新鲜标本进行超微结构观察。结果，新鲜角膜六角形细胞边界清楚，核呈淡蓝色；干燥保存角膜，六角形细胞边界不清，但核的染色及分布密度与新鲜者几无区别。电镜下，干燥保存角膜六角形镶嵌状细胞边界看不到，细胞间连接处有空泡，但细胞轮廓可见；核染色质有皱缩，但无核溶解现象。胞浆内有空泡，但仍有细胞器存在；人的细胞膜上还可看到微绒毛。结论：干燥保存角膜无活性特征虽然明显，但也存在有活性的标志。
To observe morphologically and ultrastructurally whether the preserved corneal endothelial cells were active, rabbit, monkey and human corneal endothelial cells that had been preserved for different periods of time were separately treated with 0.2% alizarin red and 0.25% trypan blue Active staining, and with normal fresh as a control. At the same time, under the electron microscope, the cornea and its fresh specimens were observed for ultrastructure. Results, fresh corneal hexagonal cells clear boundaries, the nucleus was light blue; dry preservation of the cornea, hexagonal cell boundaries are unclear, but the nucleus dyeing and distribution density and no difference between fresh and few. Electron microscopy, dry hexagonal corneal cells preserved corneal borders do not see the cell junction vacuoles, but the cell outline can be seen; nuclear chromatin shrinkage, but no nuclear dissolution phenomenon. There are vacuoles in the cytoplasm, but still there are organelles; human cells can see microvilli. Conclusion: Although the characteristics of dry preserved corneal inactivity are obvious, there are also active signs.