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考察温度对鱼类低氧耐受生化反应的影响,以白鲢(Hypophthalmichthys molitrix)幼鱼为对象;分别在15、30℃条件下驯化2周;随后将实验鱼置于密闭呼吸室中通过鱼体自发耗氧进行低氧处理,并以不经历急性低氧处理的实验鱼为对照组;分别测定实验鱼的血液和白肌中乳酸、白肌和肝脏中的糖原、葡萄糖含量。实验结果显示,经急性低氧处理后高、低温组血乳酸含量均显著上升(p<0.05),而低氧处理后15℃下肌糖原和30℃下肝葡萄糖含量却显著降低(p<0.05);随着温度的升高(不论低氧处理与否)肌乳酸含量均显著降低(p<0.05),而温度的升高并未改变实验鱼的肌葡萄糖以及肝葡萄糖的含量;统计检验结果表明温度与低氧处理间不存在交互作用。研究表明,白鲢幼鱼在面临温度变化和低氧胁迫时鱼体内代谢底物和无氧代谢产物水平均产生相应的变化,这种变化对实验鱼适应不同温度环境及低氧胁迫有着重要的意义。
The effects of temperature on the biochemical reactions of fish to hypoxia tolerance were studied. The juveniles of Hypophthalmichthys molitrix were taken as the targets. The animals were acclimated for two weeks at 15 and 30 ℃, respectively. The fish were then placed in a closed breathing chamber, Body oxygenated oxygen spontaneous hypoxia treatment, and not undergoing acute hypoxia treatment of experimental fish as a control group; were measured in the experimental fish blood and white muscle lactate, white muscle and liver glycogen, glucose content. The results showed that the blood lactic acid content increased significantly in hypobaric hypoxia group (p <0.05) after acute hypoxia treatment, but was significantly decreased in hypoglycemic muscle at 15 ℃ and hepatic glucose content at 30 ℃ (p < 0.05). With the increase of temperature (no matter hypoxia treatment or not), the content of muscle lactate was significantly decreased (p <0.05), while the increase of temperature did not change the content of muscle glucose and hepatic glucose in experimental fish; statistical test The results show that there is no interaction between temperature and hypoxia treatment. Studies have shown that silver carp juveniles in the face of temperature changes and hypoxia stress in the fish body metabolites and anaerobic metabolite levels are corresponding changes in the changes of the experimental fish to adapt to different temperature environments and hypoxia stress is important significance.