论文部分内容阅读
植物叶片中,表皮蜡质对植物的生长发育和适应外界环境起着重要作用。本研究针对拟南芥(Arabidopsis thaliana)已发表的蜡质相关基因信息,基于生物信息学的方法获得以甘蓝型油菜(Brassica napus)品种中双11号为参考的10个蜡质相关基因的44个拷贝序列。然后,以在抽薹期前后叶片蜡质差异明显的3对自然突变的甘蓝型油菜(L1050,P144和W481)为材料,利用同源克隆的方法获得这些拷贝成员在3对甘蓝型油菜中的核苷酸和蛋白序列,通过多重比对分析发现,甘蓝型油菜蜡质基因(wax inducer 2,WIN2)家族中的Bn WIN2C01在核苷酸与氨基酸水平上均存在稳定的突变位点。利用q RT-PCR分析基因Bn WIN2C01在甘蓝型油菜突变体材料抽薹期前后叶片中的表达情况,结果表明该基因在表皮蜡质合成前后的叶片中达到极显著差异水平(P<0.01),说明Bn WIN2C01基因的特异性表达可能影响了叶片蜡质的合成。本研究将有助于阐明叶表皮蜡质形成过程的分子机理,为提高油菜抗逆性提供一定的参考依据。
Plant leaves, epidermal wax on plant growth and development and adapt to the external environment plays an important role. In this study, based on the published waxy related gene information of Arabidopsis thaliana, 10 waxy related genes with reference to Shuang 11 in Brassica napus were obtained based on bioinformatics methods A copy of the sequence. Then three pairs of naturally mutated Brassica napus (L1050, P144 and W481) with obvious differences in leaf wax content before and after bolting stage were used as materials for homologous cloning of three copies of the nucleus in Brassica napus The nucleotide and amino acid sequences of Bn WIN2C01 in the wax inducer 2 (WIN2) family of Brassica napus were detected by multiple alignment analysis. The results of q RT-PCR analysis of the expression of Bn WIN2C01 in the leaves of the mutant of Brassica napus showed that there was a significant difference (P <0.01) among the leaves before and after epidermal wax synthesis The specific expression of Bn WIN2C01 gene may affect the leaf wax synthesis. This study will help elucidate the molecular mechanism of leaf epidermal wax formation and provide some references for improving rapeseed resistance.