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Objective The main purpose of this study was to investigate whether the folliculo stellate cells (FSC) respond to angiotensin(Ang)Ⅱ by increasing intracellular free Ca 2+ concentration ([Ca 2+ ]i),and where the origin of Ca 2+ mobilization is if that has occurred.Methods Pituitary cells in primary culture were prepared from male Wister rats(250g) by a conventional method and cultured in MEM supplemented with 4% normal rat serum.After 2 days in culutre,cells were loaded with 1 μmol/L fura PE3/AM for 1 h and subjected to a Ca 2+ imaging experiment with Quanti Cell 700 system.Excitation wavelengths of 340 and 380 nm were selected by means of a computer controlled filterwheel.Results The [Ca 2+ ]i of FSC in the rat anterior pituitary was elevated by Ang Ⅱ.The elevation of [Ca 2+ ]i of FSC induced by 0.1,1.0,10 and 100 nmol/L Ang Ⅱ was (56.33±6.18)( ±s ),(117.07±36.07),(175.59±40.01) and (216.02±11.52) nmol/L,respectively.The increase of [Ca 2+ ]i of FSC induced by 100 nmol/L Ang Ⅱ was not influenced by the medium without Ca 2+ (0Ca),but significantly suppressed by thapsigargin(TG),an inhibitor of ATPase.The rate of responsive FSC to Ang Ⅱ (100 nmol/L) was 61.84% which was obviously higher than that of pituitary endocrine cells(43.49%).Conclusion The present experiment demonstrates that the FSC in the rat anterior pituitary responds to Ang Ⅱ by increasing [Ca 2+ ]i,which raises the possibility that Ang Ⅱ released from either lactotrophs or gonadotrophs affects FSC through paracrine mechanism.The elevation of [Ca 2+ ]i induced by Ang Ⅱ presents a dosage dependent relation, and is possibly because of the release of Ca 2+ from an intracellular Ca 2+ pool(s).Fashions of Ca 2+ release are relative to the concentration of Ang Ⅱ.The results indicate that Ang Ⅱ functions as a paracrine factor among pituitary cells including FSC.
Objective The main purpose of this study was to investigate whether the folliculo stellate cells (FSC) respond to angiotensin (Ang) Ⅱ by increasing intracellular free Ca 2+ concentration ([Ca 2+] i), and where the origin of Ca 2+ Cells were pre-incubated with male Wister rats (250g) by a conventional method and cultured in MEM supplemented with 4% normal rat serum. After 2 days in culutre, cells were loaded with 1 μmol / L fura PE3 / AM for 1 h and subjected to a Ca 2+ imaging experiment with Quanti Cell 700 system. Excitation wavelengths of 340 and 380 nm were selected by means of a computer controlled filter wheel. Results The [Ca 2+] i of FSC in the rat anterior pituitary was elevated by Ang Ⅱ was elevation of [Ca 2+] i of FSC induced by 0.1, 1.0, 10 and 100 nmol / L of Ang Ⅱ was (56.33 ± 6.18) ± s, (117.07 ± 36.07), (175.59 ± 40.01) and (216.02 ± 11.52) nmol / L, respectively of [Ca 2+] i of FSC induced by 100 nmol / L Ang Ⅱ was not affected by the medium without Ca 2+ (0Ca), but significantly suppressed by thapsigargin (TG), an inhibitor of ATPase. To The Ang Ⅱ (100 nmol / L) was 61.84% which was obviously higher than that of the pituitary endocrine cells (43.49%). Conclusion The present experiment demonstrates that the FSC in the rat anterior pituitary responds to Ang Ⅱ by increasing [Ca 2+ ] i, which raises the possibility that Ang Ⅱ released from either lactotrophs or gonadotrophs affects FSC through paracrine mechanism. elevation of [Ca 2+] i induced by Ang Ⅱ presents a dosage dependent relation, and possibly because of the release of Ca 2+ from an intracellular Ca 2+ pool (s). Fashions of Ca 2+ release are relative to the concentration of Ang Ⅱ. The results that Ang Ⅱ functions as a paracrine factor among pituitary cells including FSC.