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目的:苦丁茶熊果酸的抑制鼻咽癌细胞的作用。方法:自苦丁茶的老叶中提取熊果酸并对其进行纯化与鉴定;使用不同浓度的熊果酸作用在已培养好的鼻咽癌细胞内,分别对24h、48h、72h、96h及120h后使用四甲基偶氮唑蓝比色法对熊果酸对鼻咽癌细胞的抑制作用情况进行检测,并采取用流式细胞术分析细胞周期和其死亡率。结果:浓度不同的熊果酸在不同时间段内对鼻咽癌细胞的抑制作用均较为明显;经流式细胞术结果表明,熊果酸阻滞鼻癌细胞于G0/G1期。结论:熊果酸对于鼻咽癌细胞的抑制作用明显,可有效的阻滞癌细胞,诱导细胞死亡。
Objective: Kudingcha Ursolic acid inhibits the function of nasopharyngeal carcinoma cells. Methods: Ursolic acid was extracted from the old leaves of Kudingcha and its purification and identification were performed. Ursolic acid was used in different concentrations of cultured urophages in cultured nasopharyngeal carcinoma cells, respectively. The cells were harvested at 24h, 48h, 72h, 96h And 120h after the use of tetramethylzirconyl blue colorimetric inhibition of ursolic acid on nasopharyngeal carcinoma cells were detected by flow cytometry and analysis of cell cycle and its mortality. Results: The ursolic acid with different concentrations inhibited nasopharyngeal carcinoma cells in different time. The results of flow cytometry showed that ursolic acid blocks the cells in G0 / G1 phase. Conclusion: Ursolic acid has obvious inhibitory effect on nasopharyngeal carcinoma cells, which can effectively block cancer cells and induce cell death.