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目的:建立青翘片中阿司品林含量测定方法。方法:色谱柱:Shim-pack CLC C_(18)(150 mm×4.6 mm,5μm);流动相:乙腈:0.08 mol·L~(-1)醋酸钠溶液(冰醋酸调pH至3.65)(15:85),流速:2 ml·min~(-1),检测波长:230 nm。紫外分光光度法检测波长:300 nm。结果:高效液相法:阿司匹林的线性范围为2～10μg·ml~(-1)(r=0.9999),回收率100.5%,RSD为0.8%(n= 6)。紫外分光光度法:阿司匹林在5～30μg·ml~(-1)呈线性,平均回收率为100.9%,RSD为0.6%(n=5)。结论:两种方法均适用于青翘片中阿司匹林的含量测定。
Objective: To establish a method for determination of aspirin in Qingqiao tablets. METHODS: The chromatographic column was Shim-pack CLC (18) (150 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile: 0.08 mol·L -1 sodium acetate solution : 85), flow rate: 2 ml · min ~ (-1), detection wavelength: 230 nm. UV spectrophotometry detection wavelength: 300 nm. Results: The HPLC method showed that the linear range of aspirin was 2 ~ 10μg · ml ~ (-1) (r = 0.9999), the recovery was 100.5% and the RSD was 0.8% (n = 6). UV spectrophotometry: Aspirin was linear at 5 ~ 30μg · ml ~ (-1) with an average recovery of 100.9% and a RSD of 0.6% (n = 5). Conclusion: Both methods are suitable for the determination of aspirin in Qingqiao Tablets.