利用铜绿假单胞菌(Pseudomonas aeruginosa TX16)细胞催化阿糖2,6-二氨基嘌呤核苷进行脱氨反应转化生成阿糖鸟苷。经优化得到的最适酶促反应条件为:在160 mmol/L磷酸盐缓冲液(p H 7.5)中加入13%铜绿假单胞菌TX16细胞和30 mmol/L阿糖2,6-二氨基嘌呤核苷,反应温度60℃,反应120 h。在上述条件下,嘌呤核苷的转化率可达90.0%。 The use of Pseudomonas aeruginosa TX16 cells catalyzed deaza-conversion of arabinose 2,6-diaminopurine to produce guanosine. Optimum conditions for the optimal enzymatic reaction were as follows: adding 13% Pseudomonas aeruginosa TX16 cells and 30 mmol / L arabinose 2,6-diamino group in 160 mmol / L phosphate buffer (p H 7.5) Purine nucleoside, the reaction temperature is 60 ℃, the reaction 120 h. Under the above conditions, purine nucleoside conversion rate of up to 90.0%.