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单纯疱疹病毒(HSV)经过一个急性增殖期后,可以以潜伏的方式在三叉神经节存在;然而,对于这种现象的机制却了解甚少。作者通过用HSV感染小鼠单侧神经节后,定期杀死小鼠,分别取出感染侧和对侧神经节,制成匀浆,测定接种后不同时间病毒含量、干扰素和干扰素所诱导的2′,5′—寡腺苷化物(2′,5′—A)合成酶的活性及血清中HSV抗体的水平,从而探讨病毒从急性增殖相向潜伏相转化的机制。单纯疱疹病毒VR—3株在Vero细胞中传代三次,用1.2~2.0×10~8PFu/毫升病毒悬液0.05毫升,以
Herpes Simplex Virus (HSV) can survive in the trigeminal ganglion after an acute proliferative phase; however, the mechanism for this phenomenon is poorly understood. After infecting the unilateral ganglion of mice with HSV, the mice were sacrificed on a regular basis, and the infected and contralateral ganglia were removed and homogenized respectively. The viral contents induced by interferon and interferon were measured at different time points after inoculation 2 ’, 5’-oligoadenylate (2’, 5’-A) synthase activity and serum levels of HSV antibodies in order to explore the mechanism of virus transformation from acute proliferative phase to latent phase. The herpes simplex virus VR-3 strain was subcultured in Vero cells three times with 0.05 ml of 1.2-2.0 x 10-8 PFU / ml virus suspension to