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目的:研究组蛋白去乙酰化酶抑制剂曲古菌素A(trichostatin A,TSA)诱导急性髓性白血病细胞表达共刺激分子CD80和CD86。方法:流式细胞仪检测TSA诱导HL-60细胞、急性髓性白血病患者单个核细胞表达共刺激分子CD80和CD86及细胞活力;RT-PCR法分析CD80和CD86 mRNA的表达情况;75Gy(gray,Gy)照射TSA诱导的HL-60细胞后,采用CCK-8法检测共刺激分子表达增高后,HL-60细胞对健康人单核细胞的增殖作用。结果:TSA使HL-60细胞CD86的表达上调,也可急性髓性白血病患者单核细胞CD86的表达上调,TSA诱导HL-60细胞表达共刺激分子CD86后,可促进健康人单核细胞的增殖。结论:TSA诱导HL-60细胞、急性髓性白血病患者单核细胞共刺激分子CD86表达增高,促进健康人单核细胞的增殖。
AIM: To investigate the effects of histone deacetylase inhibitor trichostatin A (TSA) on the expression of costimulatory molecules CD80 and CD86 in acute myeloid leukemia cells. Methods: Flow cytometry was used to detect the expression of costimulatory molecules CD80 and CD86 and the viability of mononuclear cells in HL-60 cells and acute myeloid leukemia cells by flow cytometry. The expression of CD80 and CD86 mRNA was analyzed by RT-PCR. Gy), the proliferation of HL-60 cells was detected by CCK-8 assay. Results: TSA up-regulated the expression of CD86 in HL-60 cells and up-regulated the expression of monocyte CD86 in patients with acute myeloid leukemia. TSA induced the proliferation of monocytes in HL-60 cells after it expressed costimulatory molecule CD86 . CONCLUSIONS: TSA induces an increase in monocyte costimulatory molecule CD86 in HL-60 cells and in patients with acute myeloid leukemia and promotes the proliferation of healthy human monocytes.