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目的探讨细胞外基质金属蛋白酶诱导因子(extracellular matrix metalloproteinase inducer,EMMPRIN)、基质金属蛋白酶9(matrix metalloproteinases-9,MMP-9)和P38丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)在子宫内膜异位症(endometriosis,EMs)中的表达及相关性。方法收集2008年5-12月重庆医科大学附属第一医院收治的EMs患者44例(异位内膜44例,在位内膜38例),对照组为同期非EMs患者在位内膜34例。应用免疫组化SABC三步法检测各组织中EMMPRIN、MMP-9、P38及磷酸化P385(p-P38)蛋白的表达,并对各蛋白表达水平进行相关性分析。结果 EMMPRIN、MMP-9、P38、p-P38蛋白在EMs异位内膜表达均高于EMs在位内膜组及对照组在位内膜,差异有统计学意义(P<0.05);Spearman相关分析显示,在EMs异位内膜中各蛋白表达呈正相关(P<0.05)。在EMs异位内膜组中各蛋白表达Ⅲ～Ⅳ期高于Ⅰ～Ⅱ期,差异均有统计学意义(P<0.05)。EMs在位内膜组与对照组相比,各蛋白表达差异无统计学意义(P>0.05)。结论 EMMPRIN、MMP-9在EMs异位内膜中呈显著高表达并呈正相关性,在EMs的发生发展过程中可能起重要作用。EMMPRIN诱导刺激MMP-9高表达可能与p38 MAPK途径有关。
Objective To investigate the expression of extracellular matrix metalloproteinase inducer (EMMPRIN), matrix metalloproteinases-9 (MMP-9) and mitogen-activated protein kinase (MAPK) Expression and Correlation in Endometriosis (EMs). Methods Forty-four EMs (ectopic endometrium, 44 cases, eutopic endometrium, 38 cases) were collected from the First Affiliated Hospital of Chongqing Medical University from May to December in 2008. The control group consisted of 34 cases of eutopic endometrium . Immunohistochemical SABC three-step method was used to detect the expression of EMMPRIN, MMP-9, P38 and phosphorylated P385 (p-P38) in each tissue, and correlation analysis of the expression of each protein. Results The expressions of EMMPRIN, MMP-9, P38 and p-P38 in ectopic endometrium in EMs were significantly higher than those in EMs eutopic endometrium and control group (P <0.05), Spearman correlation Analysis showed that there was a positive correlation between the expression of each protein in EMs ectopic endometrium (P <0.05). In EMs ectopic endometrium group, the protein expression of stage Ⅲ ~ Ⅳ was higher than that of stage Ⅰ ~ Ⅱ, the differences were statistically significant (P <0.05). EMs eutopic endometrium group compared with the control group, the difference was not statistically significant (P> 0.05). Conclusion EMMPRIN and MMP-9 are highly expressed and positively correlated in the ectopic endometrium of EMs, which may play an important role in the development of EMs. The EMMPRIN-induced stimulation of MMP-9 overexpression may be related to the p38 MAPK pathway.