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目的建立同时测定繁枝补血草中槲皮苷和槲皮素含量的HPLC法。方法采用Agilent C18色谱柱(250mm×4.6mm,5.0μm);流动相为甲醇-4mL·L-1磷酸水溶液(50∶50);流速为1.0mL·min-1;检测波长为360nm;柱温为25℃;进样量为10μL。用外标法计算其含量。结果槲皮苷质量浓度在4.912~98.24μg·mL-1范围内与峰面积有良好的线性关系,回归方程y=30.434x-11.864(r=0.999 6);槲皮素质量浓度在12.833~102.667μg·mL-1范围内与峰面积有良好的线性关系,回归方程y=43.358x-45.586(r=0.999 7)。槲皮苷的平均回收率为100.73%,RSD值为1.82%(n=6);槲皮素的平均回收率为100.47%,RSD值为1.35%(n=6)。结论该方法操作简便、快速、准确、误差小、重复性好,可作为繁枝补血草中槲皮苷和槲皮素的定量分析方法。
Objective To establish an HPLC method for the simultaneous determination of quercetin and quercetin in Methods The mobile phase consisted of methanol-4mL·L-1 phosphoric acid solution (50:50), the flow rate was 1.0mL · min-1, the detection wavelength was 360nm, the column temperature (250mm × 4.6mm, 5.0μm) 25 ℃; injection volume of 10μL. External standard method to calculate its content. Results The calibration curve of quercetin was linear with the peak area in the range of 4.912-98.24μg · mL-1. The regression equation y = 30.434x-11.864 (r = 0.999 6). The concentration of quercetin was between 12.833 and 102.667 μg · mL-1, the regression equation y = 43.358x-45.586 (r = 0.999 7). The average recoveries of quercetin were 100.73% and the RSD was 1.82% (n = 6). The average recoveries of quercetin were 100.47% and RSD was 1.35% (n = 6). Conclusion The method is simple, rapid, accurate, small error, good repeatability, and can be used as a quantitative method for the determination of quercetin and quercetin in.