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为探讨温光敏雄性不育小麦BNS育性与内源激素的关系,该研究以BNS不育株和可育株的叶片、幼穗和花药为研究材料(以鲜重计),在雌雄蕊分化期(Ⅰ)到三核期(Ⅵ)之间分期取材。采用酶联免疫法测定生长素(IAA)、赤霉素(GA)和脱落酸(ABA)的含量。结果显示:(1)在发育过程中,不育株IAA含量(33～95ng/g)显著低于可育株(57～112ng/g);不育株ABA、GA含量分别为48～129ng/g、3～14ng/g,可育株分别为77～132ng/g、4～11ng/g,大部分时期不育株显著低于可育株。(2)在雌雄蕊分化期,幼穗不育株IAA和GA含量分别为33.85ng/g和7.13ng/g,可育株分别为50.55ng/g和11.84ng/g,不育株IAA和GA含量均显著低于可育株。(3)在幼穗发育期内,不育株IAA/ABA、IAA/GA和ABA/GA分别为0.4～1.1、4.3～15和7～20,可育株为0.5～0.9、4.3～11.5和7.3～13,与可育株相比,不育株中激素比例浮动较大,表现比例失调。研究表明,BNS幼穗发育中激素的比例失调是雄性不育的形成原因之一,而雌雄蕊分化期的IAA、GA的含量下降可能促进BNS雄性不育的发生。
In order to investigate the relationship between BNS fertility and endogenous hormones in the thermo-sensitive male sterile wheat (Triticum aestivum L.), leaf, young spike and anther of BNS sterile and fertile plants were used as materials (fresh weight) Period (Ⅰ) to the trinuclear phase (Ⅵ) staging drawn. The contents of IAA, GA and ABA were determined by enzyme-linked immunosorbent assay. The results showed that: (1) IAA content (33-95ng / g) of sterile plants was significantly lower than that of fertile plants (57- 112ng / g) during the developmental stages; ABA and GA of sterile plants were 48-129ng / g, 3 ~ 14ng / g, fertile plants were 77 ~ 132ng / g, 4 ~ 11ng / g, most of the sterile plants were significantly lower than the fertile plants. (2) The IAA and GA contents of young sterile plants were 33.85ng / g and 7.13ng / g, respectively. The fertile plants were 50.55ng / g and 11.84ng / g, respectively. The sterile plants IAA and GA GA content were significantly lower than fertile plants. (3) The IAA / ABA, IAA / GA and ABA / GA of sterile lines were 0.4-1.1, 4.3-15 and 7-20 respectively during the panicle growth stage, and the fertile plants were 0.5-0.9 and 4.3-1.5 7.3 ~ 13. Compared with fertile plants, the ratio of hormones in sterile plants fluctuated a lot, showing imbalance. Studies have shown that the imbalance of hormones in panicle development of BNS is one of the reasons for the formation of male sterility. However, the decrease of IAA and GA in pistil and stamen may promote the development of male sterile in BNS.