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目的探讨肝X受体α(LXRα)在大鼠新月体型肾小球肾炎(GN)的表达及意义。方法 7周龄Wistar Kyoto雄性大鼠40只随机分为两组。其中20只静脉注射兔抗大鼠肾小球基底膜抗体构建新月体型肾小球肾炎模型,20只作为正常对照。于注射抗体后第3、7、14、28和49天分别测定24h尿蛋白;免疫组织化学法检测第3、14、28和49天肾炎组和对照组大鼠肾组织LXRα表达;Western blotting和实时定量PCR检测第14天肾炎肾皮质LXRα蛋白和mRNA表达变化。结果注射抗体后第14天尿蛋白达(167.03±42.50)mg/24h;新月体百分比达65%;肾炎大鼠肾小管上皮细胞核LXRα表达明显增加;肾炎组肾皮质LXRα蛋白表达明显高于同期对照组,但肾皮质mRNA表达则未见上调。结论新月体型肾炎肾小管上皮细胞核LXRα蛋白表达增强,说明肾小管LXR通路参与此型肾炎的发病过程。
Objective To investigate the expression of liver X receptor α (LXRα) in crescentic glomerulonephritis (GN) of rats and its significance. Methods Forty Wistar Kyoto male rats, 7 weeks old, were randomly divided into two groups. Twenty rabbits were injected intravenously with rabbit anti-rat glomerular basement membrane antibody to construct crescentic glomerulonephritis model, 20 were used as normal control. 24h urinary protein were detected on the 3rd, 7th, 14th, 28th and 49th day after the injection of the antibody. The expression of LXRα in the nephritic group and the control group on the 3rd, 14th, 28th and 49th day were detected by immunohistochemical method; Western blotting and Real-time quantitative PCR was used to detect the protein and mRNA expression of LXRα in nephritis and renal cortex on the 14th day. Results Urine protein was (167.03 ± 42.50) mg / 24h on the 14th day after injecting the antibody, and the percentage of crescent was 65%. The expression of LXRα in renal tubular epithelial cells of nephritis rats was significantly increased. Control group, but renal cortex mRNA expression was not up-regulated. Conclusions The expression of LXRα protein in renal tubular epithelial cells of crescentic nephritis is increased, indicating that renal tubular LXR pathway is involved in the pathogenesis of this nephritis.