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目的建立高效液相色谱(HPLC)紫外检测器测定T-2毒素的方法,并评价该方法的可操作性,最终推广到下级单位使用。方法采集哈尔滨市市售大米,经过粉碎后,染毒备用。取染毒后大米粉末,加入乙腈水溶液及正己烷萃取,涡旋混合,离心后,移取己烷层氮气吹干,残渣加人甲醇水溶解进样。色谱条件:采用C18常规反相色谱柱(150 mm×4.6 mm,5μm),流动相为甲醇∶水(7∶3),紫外检测波长208 nm,柱温为室温,流速0.5 ml/min。结果 T-2毒素的保留时间为11.6 min;在50~800 ng范围内线性关系良好;样品中T-2毒素的平均回收率为93.00%。结论该方法测定T-2毒素含量,结果准确,可用于粮食中T-2毒素的含量测定。
OBJECTIVE To establish a method for the determination of T-2 toxin by UV detector with high performance liquid chromatography (HPLC) and to evaluate the operability of this method and to eventually promote it to subordinate units. Methods Harvested rice on the market in Harbin City, after crushing, arsenic poisoning reserve. Take the rice powder after poisoning, add acetonitrile aqueous solution and n-hexane extraction, vortex and mix. After centrifugation, remove the hexane layer and dry with nitrogen. The residue is dissolved in methanol to dissolve the sample. Chromatographic conditions: C18 conventional reversed-phase column (150 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of methanol and water (7: 3). The UV detection wavelength was 208 nm. The column temperature was at room temperature and the flow rate was 0.5 ml / min. Results The retention time of T-2 toxin was 11.6 min. The linearity was good in the range of 50 to 800 ng. The average recovery of T-2 toxin in the sample was 93.00%. Conclusion The method for the determination of T-2 toxin content with accurate results can be used for determination of T-2 toxin content in food.