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目的制备银溶胶表面增强拉曼散射(surface-enhanced Raman scattering,SERS)活性基底,并基于自行制备的基底检测米线中的乌洛托品。方法利用扫描电子显微镜(SEM)和紫外-可见吸收光谱对制备的银纳米颗粒进行表征。采用氯化钠作为促凝剂,对所制备的活性基底进行性能优化。基于自行制备的SERS活性基底,研究了乌洛托品的表面增强拉曼光谱,并将这种检测方法应用于米线中乌洛托品的检测。结果当乌洛托品的浓度达到1 mg/kg时,仍然可以得到明显的拉曼信号,乌洛托品在1~5 mg/kg的范围内呈现良好的线性关系,R2=0.928。结论 SERS技术操作简便、快速、准确,可用于米线中痕量乌洛托品的现场快速检测。
Objective To prepare surface-enhanced Raman scattering (SERS) -based silver sols and to detect urotropine in rice noodles based on self-prepared substrates. Methods The prepared silver nanoparticles were characterized by scanning electron microscopy (SEM) and ultraviolet-visible absorption spectroscopy. Sodium chloride was used as coagulant to optimize the prepared active matrix. Based on the SERS active substrate prepared by ourselves, the surface enhanced Raman spectroscopy of urotropine was studied, and this method was applied to the detection of urotropin in rice noodles. Results When the concentration of urotropin reached 1 mg / kg, obvious Raman signal was still obtained. Urotropine showed a good linearity in the range of 1 ~ 5 mg / kg, R2 = 0.928. Conclusion The SERS technique is simple, rapid and accurate and can be used for rapid on-site detection of trace urotropine in rice noodles.