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目的研究河南省南阳市2012-2014年手足口病流行期间健康人群肠道病毒(Enterovirus,EV)感染状况。方法采用体外反转录-聚合酶链反应(reverse transcriptase-polymerase chain reaction,RT-PCR),对南阳市2012 2014年分离自健康人群的EV进行核酸检测。调查对象共分为10个年龄组,每组10人,每年调查100人,每人均采集粪便和咽拭子标本。结果在连续3年流行期监测的300名健康人群中,肠道病毒感染率为42.67%。≤5岁儿童为主要感染人群,性别分布差异无统计学意义(χ2=0.587,P>0.05)。结论肠道病毒在健康人群中分布普遍,以其他EV为主,未检出肠道病毒71型和柯萨奇病毒A组16型。
Objective To investigate the status of Enterovirus (EV) infection in healthy population during the period of HFMD in 2012-2014 in Nanyang City, Henan Province. Methods The nucleic acid of EV isolated from healthy population in Nanyang from 2012 to 2014 was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Subjects were divided into 10 age groups, 10 in each group, 100 people were surveyed each year, each collecting feces and throat swab specimens. Results The prevalence of enterovirus infection was 42.67% among 300 healthy people monitored during the three-year epidemic period. Children less than 5 years old were the main infection groups. There was no significant difference in gender distribution between the two groups (χ2 = 0.587, P> 0.05). Conclusions Enterovirus is widely distributed in healthy population. Other EVs are dominant. No enterovirus 71 and Coxsackievirus A 16 were detected.