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目的以咖啡因为代谢探针 ,研究膀胱癌发生发展的N_乙酰化代谢表型分布的统计学相关性。方法根据人尿液中咖啡因代谢物5 -乙酰氨基_6_甲酰氨基_3_甲基尿嘧啶(AFMU)和甲磺嘌呤1X)的峰高比值绘制概率分布直方图和概率单位图 ,寻找区分快慢乙酰化代谢表型的截点 ,确定人的乙酰化代谢表型分布。结果健康志愿者和膀胱癌患者概率分布直方图和概率单位图呈明显多态性 ,截点为1 10 ,即大于1.10为快乙酰化代谢表型 ,小于1.10为慢乙酰化代谢表型。健康志愿者中快、慢人乙酰化表型分别为149例(73 7 %)和54例(26 3 %)。膀胱癌患者分别为36例(53 7 %)和31例(46 3) ,两者存在显著统计学差异(P<0.01)。志愿者和膀胱癌患者基因频率分别为0.51和0 68 ,优势比为2 376(95 %可信区间为1 3513和4 1776)。结论中国人乙酰化代谢表型分布呈多型性慢乙酰化代谢表型个体可能为膀胱癌多发和易感人群
Objective To study the statistical correlation of N-acetylated metabolic phenotype distribution in bladder cancer with caffeine as a metabolic probe. Methods Based on the ratio of peak height to peak height of caffeine metabolites 5 - acetylamino - 6 - carboxamido - 3 - methyluracil (1 ×) in human urine, the probability distribution histograms and probability units were plotted. Look for the cut-off point that distinguishes between quick and slow acetylated metabolic phenotypes and determine the human acetylated metabolic phenotype distribution. Results The probability distribution histogram and probability unit map of healthy volunteers and bladder cancer patients were significantly polymorphic with a cutoff point of 10, ie, greater than 1.10 for the rapid acetylated metabolic phenotype and less than 1.10 for the slow acetylated metabolic phenotype. In healthy volunteers, the acetylated phenotypes of fast and slow persons were 149 (73.7%) and 54 (26.3%), respectively. There were 36 cases (53.7%) and 31 cases (463) of bladder cancer with significant difference (P <0.01). The frequencies of genes in volunteers and bladder cancer were 0.51 and 0 68, respectively, and the odds ratio was 2 376 (95% confidence interval was 1 3513 and 4 1776). Conclusion The Chinese people with acetylated metabolic phenotype showed polymorphic slowly acetylated phenotypic individuals may be multiple bladder cancer and susceptible populations