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地鼠肾细胞狂犬病疫苗原液经１００ ｋＤ 膜浓缩 ３０ 倍，分别选用（１）ＤＥＡＥ Ｓｅｐｈａｒｏｓｅ ＣＬ－６Ｂ离子交换层析法；（２）Ｓｅｐｈａｃｒｙ１ Ｓ－２００ ＨＲ 分子筛选层析法；（３）二次蔗糖等密度区带离心法对其进行纯化。用此三种方法各试制３ 批精制疫苗，结果表明，经ＤＥＡＥ Ｓｅｐｈａｒｏｓｅ ＣＬ－６Ｂ离子交换层析纯化后疫苗总蛋白含量减少９９％ 以上，抗原比活性提高１５９ 倍，抗原回收率达５０％ ，纯化疫苗以ＮＩＨ 法效力测定平均为５．４ ＩＵ／２ｍ ｌ；经Ｓｅｐｈａｃｒｙ１ Ｓ－２００ＨＲ 分子筛层析纯化后疫苗总蛋白含量减少 ９８％ 以上，抗原比活性提高４１ 倍，抗原回收率达６３％ ，纯化疫苗效力平均为６．２５ ＩＵ／２ｍ ｌ；经一次蔗糖等密度区带离心法纯化后疫苗总蛋白含量减少９８％ 以上，抗原比活性提高３２１ 倍，抗原回收率达４３％ ，纯化疫苗效力平均为６．１８ ＩＵ／２ｍ ｌ，三种纯化疫苗均符合Ｗ ＨＯ 规程要求。
The hamster kidney cell rabies vaccine stock solution was concentrated 30-fold by 100 kD membrane, respectively, (1) DEAE Sepharose CL-6B ion exchange chromatography; (2) Sephacryl S-200 HR molecular sieve chromatography; (3) Sucrose and other isolates were purified by centrifugation. Three batches of the purified vaccine were tested by these three methods. The results showed that the total protein content of the vaccine purified by DEAE Sepharose CL-6B ion exchange chromatography was reduced by more than 99%, the specific activity of the antigen was increased by 159 times and the antigen recovery rate was 50% The purified vaccine had an average of 5.4 IU / 2 ml measured by NIH method. The total protein content of the vaccine purified by Sephacryl S-200HR chromatography was reduced by more than 98%, the specific activity of the antigen was increased by 41 times and the antigen recovery rate was 63% The average potency of the purified vaccine was 6.25 IU / 2m l. The total protein content of the vaccine was reduced by more than 98% after a single centrifugation in sucrose density zone, the specific activity of the antigen was increased by 321 times and the antigen recovery rate was 43% With an average of 6.18 IU / 2m l. All three purified vaccines met the W HO protocol.