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近年来,我们根据中医脾胃学说理论。用健脾灵片治疗慢性溃疡性结肠炎取得满意疗效。为了探讨中医脾胃的实质和健脾灵治疗本病的机理,我们用免疫法复制成大鼠溃疡性结肠炎动物模型,并用健脾灵进行复健治疗观察。现将研究结果报告如下。材料和方法一、实验材料: 1、实验动物;系哈医大实验动物学部所提供的wistar雄性大白鼠,体重120克左右,共100只,实验期间饲以定量配方饮食,自由饮水。 2、抗原制备:取人体术后新鲜结肠,制成结肠粘膜匀浆,冷冻24小时,融冻后以3000
In recent years, we are based on theories of spleen and stomach theory in Chinese medicine. Treating chronic ulcerative colitis with Jianpiling Tablets has achieved satisfactory results. In order to explore the essence of spleen and stomach and the mechanism of Jianpiling in treating this disease, we used an immunological method to replicate an animal model of rat ulcerative colitis, and took Jianpiling for rehabilitation treatment. The results of the study are reported below. Materials and Methods 1. Experimental materials: 1. Experimental animals; Wistar male rats provided by the Department of Experimental Animal Science of Harbin Medical University, weighing about 120 grams, and 100 in total. During the experiment, they were fed a quantitative formula diet and were allowed to drink freely. 2. Preparation of antigen: Take fresh colon from human body, make homogenate of colon mucosa, freeze for 24 hours, thawed to 3000