论文部分内容阅读
目的检测CIAPIN1mRNA在结肠癌组织及正常结肠粘膜组织中的表达水平,研究CIAPIN1基因与结肠癌发生的相关性。方法收集28例结肠癌患者手术后的癌组织及切缘旁>5cm的正常粘膜组织标本(术前未行放化疗),采用反转录PCR法检测28例结肠癌患者结肠癌组织、正常结肠粘膜组织的CIAPIN1mRNA表达水平。结果 28例结肠癌患者癌组织CIAPIN1mRNA阳性表达率为10.7%,正常结肠粘膜组织阳性表达率为100%,差异有统计学意义(P=0.000)。结论 CIAPIN1mRNA在结肠癌组织的表达缺失或降低可能与结肠癌的发生相关。
Objective To detect the expression of CIAPIN1 mRNA in colon cancer tissues and normal colonic mucosa and to study the correlation between CIAPIN1 gene and colon cancer. Methods Twenty-eight colon cancer specimens were collected from the normal tissues of the colon cancer patients and normal mucosa specimens (> 5 cm) who had resection margins without preoperative radiotherapy and chemotherapy. Reverse transcription-polymerase chain reaction (RT-PCR) Mucosal tissue CIAPIN1 mRNA expression levels. Results The positive expression rate of CIAPIN1 mRNA in 10 cases of colon cancer tissues was 10.7% and that in normal colon mucosa tissues was 100% (P = 0.000). Conclusion The loss or decrease of CIAPIN1 mRNA expression in colon cancer may be related to the occurrence of colon cancer.