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1 引言 红景天(Rhodiola Saera (prain) Fu),为景天科多年生草本植物,分布于西藏、青海和东北的长白山地区,生长于高山岩石处,以全草入药,具有抗缺氧、抗寒冷、抗疲劳和抗微波辐射等功能,还有提高工作效率,延缓机体衰老,防治老年疾病等作用。 红景天的有效成分为红景天甙(Salidroside),过去沿用薄层色谱法测定,本文建立了高效液相色谱法,使样品处理简单省时,且具有较高的灵敏度和准确度。 2仪器与试剂 日本岛津LC-6A高效液相色谱系统,包括LC-6A高压溶剂输送泵;CTO-6A柱温箱;CLC-6B系统控制器:SPD-6AV紫外可见分光光度计;C-R4A色谱数据处理机;7125进样阀(美国产)。 红景天甙对照品系按文献制得,用甲醇稀释成合适的浓度。甲醇为优级纯,其它试剂均为分析纯,实验用水为经离子交换柱处理后又经亚沸蒸馏的纯水。
INTRODUCTION Rhodiola saera (prain) Fu is a perennial herb of Sedum, distributed in the Changbai Mountain area in Tibet, Qinghai and northeast China. It grows on alpine rocks, Cold, anti-fatigue and anti-microwave radiation and other functions, as well as improve work efficiency, delaying body aging, prevention and treatment of senile diseases and so on. Rhodiola active ingredient is salidroside (Salidroside), followed by thin-layer chromatography, the established high-performance liquid chromatography, the sample processing is simple and time-saving, and has high sensitivity and accuracy. 2 instruments and reagents Japan Shimadzu LC-6A high performance liquid chromatography system, including the LC-6A high pressure solvent pump; CTO-6A oven; CLC-6B system controller: SPD-6AV UV-visible spectrophotometer; C- R4A chromatography data processor; 7125 injection valve (made in USA). Salidroside reference strains prepared according to the literature, diluted with methanol to a suitable concentration. Methanol is excellent grade pure, other reagents are of analytical grade, experimental water after ion exchange column treated with sub-boiling distillation of pure water.