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目的:探索一种适用于初学者的以STZ法复制1型糖尿病(T1DM)大鼠模型的技术方案。方法:100只雄性Wistar大鼠随机分为模型组(DMM)和对照组(NC)。采用有别于现有文献报道的技术方案即分批次建模。DMM组分3批依次分别一次性腹腔注射60 mg/kg(DMM1)、70 mg/kg(DMM2)、55 mg/kg(DMM3)STZ溶液,NC组注射等量的柠檬酸盐缓冲液。DMM1、DMM2建模未成功者均编为DMM3。将72 h后禁食17 h空腹血糖值(FPG)≥11.1 mmol/L和相应生理指标作为成模的判断依据。结果:DMM1建模前后FPG无差异(P>0.05),DMM1建模失败;DMM2建模前后FPG具有显著差异(P<0.05),成模率为75%。DMM3补充剂量后建模成功。结论:分批次进行的STZ法建模方案适宜于初学者使用。
Objective: To explore a suitable protocol for beginners to copy type 1 diabetes mellitus (T1DM) rat model by using STZ method. Methods: One hundred male Wistar rats were randomly divided into model group (DMM) and control group (NC). Different from the existing literature reported that the technical program that is batch-modeled. The rats in DMM group were given intraperitoneal injections of 60 mg / kg DMM1, 70 mg / kg (DMM2) and 55 mg / kg (DMM3) STZ solution in 3 batches. NC group was injected with the same amount of citrate buffer solution. DMM1, DMM2 unsuccessful models are compiled as DMM3. The fasting blood glucose (FPG) ≥11.1 mmol / L after 72 h and the corresponding physiological indexes were used as the basis for judgment. Results: There was no difference in FPG before and after DMM1 modeling (P> 0.05). DMM1 failed to establish the model before and after DMM2 modeling. There was a significant difference (P <0.05) between FPG and DMM2 modeling before and after modeling. The DMM3 dose was modeled successfully. Conclusion: The STZ method in batches is suitable for beginners.