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①目的观察不同溶液对冷冻复温后精子功能的影响。②方法用４种胶体和４种晶体溶液分别孵育冷冻复温后的精子，检测孵育后精子对人宫颈粘液穿透力和不同时间的精子活动率。③结果胶体溶液孵育后１ｈ精子活动率好于晶体溶液（Ｆ＝８．７６３４，ｑ＝２．９５～５．１０，Ｐ＜０．０５），而晶体溶液中精子穿透力好于胶体溶液（Ｆ＝１１．６９０２，ｑ＝３．２３～４．６２，Ｐ＜０．０１），孵育１２ｈ后果糖和葡萄糖溶液组的精子活动率均明显高于对照组（ｑ＝７．１０，３．３４，Ｐ＜０．０１）。④结论选择使用胶、晶体溶液和果糖复合液对复温后精子进行适宜的处理，可以保持或改善精子功能状态，提高人工授精成功率。
①Objective To observe the effect of different solutions on sperm function after freezing and rewarming. Methods The sperms after cryo-rewarming were incubated with four kinds of colloids and four kinds of crystal solutions, respectively. The sperm motility of human cervical mucus and the sperm motility of human cervical mucosa after incubation were tested. Results The activity of sperm in 1h colloidal solution was better than that of crystalloid solution (F = 8.7634, q = 2.95-5.10, P <0.05), while the penetration of sperm in crystalloid solution was better than colloidal solution (F = 11.6902, q = 3.23-4.62, P <0.01). After 12 hours of incubation, the sperm motility of fructose and glucose solution groups were significantly higher than that of the control group (q = 7.10,3 .34, P <0.01). ④ Conclusion Select the use of plastic, crystal solution and fructose complex solution of sperm after rewarming appropriate treatment, can maintain or improve the functional status of sperm and improve the success rate of artificial insemination.