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目的:建立反相高效液相色谱法测定银杏达莫注射液水解前芦丁、槲皮素的含量。方法:采用Zorbax Eclipse Plus C18柱(4.6 mm×150 mm,5μm),甲醇-0.4%磷酸溶液(38∶62)为流动相,流速1.0 mL.min-1,检测波长360 nm,柱温30℃。结果:芦丁、槲皮素分别在0.046675～4.6675μg、0.019927～1.9927μg之间线性关系良好,r=0.9999;平均回收率分别为98.9%,97.8%。结论:本方法有效、简便、精确,可用于银杏达莫注射液水解前芦丁、槲皮素的含量测定。
Objective: To establish an RP-HPLC method for the determination of rutin and quercetin in Yinxingdamo injection before hydrolysis. Methods: The mobile phase was a Zorbax Eclipse Plus C18 column (4.6 mm × 150 mm, 5 μm) with methanol-0.4% phosphoric acid solution (38:62) at a flow rate of 1.0 mL · min- . Results: The linear relationship between rutin and quercetin was 0.046675 ~ 4.6675μg and 0.019927 ~ 1.9927μg respectively, r = 0.9999. The average recoveries were 98.9% and 97.8% respectively. Conclusion: The method is effective, simple and accurate, and can be used for the determination of rutin and quercetin in Yin Xing Damo injection before hydrolysis.