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小麦根腐病(Bipolaris sorokinian)毒素用减压浓缩,丙酮和正丁醇与氯仿等量混合液萃取的方法,制成棕色粘稠状的粗制品。经生物测定,粗制品与真菌培养滤液和分生孢子悬浮液对小麦叶片具有同等致病力。真菌毒素对寄主小麦有明显的毒害作用,造成种子发芽率,幼苗生长速度和植株体鲜重下降,并使根活力降低,损伤寄主组织细胞膜,造成胞内电解质外漏。真菌毒素对小麦体细胞形成愈伤组织和愈伤组织的生长发育也有很大毒害作用,降低外植体愈伤组织形成率,阻碍愈伤组织生长速度。
The Bipolaris sorokinian toxin was concentrated to a brown, viscous crude product by concentration under reduced pressure, extraction of a mixture of acetone, n-butanol and chloroform. Bioassay, crude and fungal culture filtrate and conidial suspension of wheat leaves have the same pathogenicity. Mycotoxins have significant toxic effects on host wheat, resulting in decreased seed germination rate, seedling growth rate and plant fresh weight, decreased root activity and damaged the host cell membrane, resulting in leakage of intracellular electrolytes. Mycotoxins also have a great toxic effect on the growth and development of callus and callus of somatic cells of wheat, reduce the rate of explant callus formation and hinder the growth of callus.