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以2种不同浓度(1和2mmol)的 NaN_3分别处理圭辐3号和红410的预浸种子,期望从中获得雄性不育突变。结果表明,2mmol 处理的,圭辐3号和红410的 M_2代均出现雄性不育突变体,其突变频率(按 M_1穗计)分别为0.55%和0.28%,突变体频率(按 M_2株计)分别为0.06%和0.02%。而1mmol 处理的,只有圭辐3号的 M_2代出现雄性不育突变,其突变频率和突变体频率分别为0.25%和0.05%。试验中获得的雄性不育突变体均属无花粉型,开花时花药不开裂,在人工授粉条件下结实良好,但自由授粉结实率低。遗传分析表明,其雄性不育性受一对隐性基因所控制。
The preimpregnated seeds of Gui Fu No.3 and Red 410 were treated with NaN 3 at two different concentrations (1 and 2 mmol), respectively, and the male sterility mutation was expected to be obtained. The results showed that male sterility mutants were found in the 2 mmol treatment of the M_2 generations of Guifei 3 and Hong 410 with the mutation frequencies of 0.55% and 0.28%, respectively. The frequency of the mutant ) Were 0.06% and 0.02% respectively. However, only the male sterile line M_2 of Guifei 3 was treated with 1 mmol, and its mutation frequency and mutation frequency were 0.25% and 0.05% respectively. Male sterile mutant obtained in the experiment are non-pollen type anthers flowering is not cracking, artificial pollination conditions, good, but the low rate of free pollination. Genetic analysis showed that male sterility was controlled by a pair of recessive genes.