Screening of an Endophyte Producing Ginkgo biloba L. Flavonoids

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[Objective]To protect the Ginkgo biloba L.resources,and to find the endophyte producing G.biloba flavonoids.[Method]With G.biloba leaves and roots as the plant materials,tissue block isolation method and streaking cultivation method were adopted for the isolation and purification of endophytes.With extracting solution of G.biloba flavonoids as the reference,TLC analysis on fermentation liquids was conducted.Al(NO 3) 3-NaNO 2 was used as the specific chromogenic agent of flavonoids.[Result] A total of eight kinds of endophytes were screened.TLC analysis showed that the color band or spot of 07-Y8 fermentation liquid had the same color and similar mobility as the standard extracting solution of G.biloba flavonoids.[Conclusion] Strain 07-Y8 might have the capability of being fermented into G.biloba flavonoids or other similar compounds,which provided new approach for the development and utilization of G.biloba flavonoids. [Objective] To protect the Ginkgo biloba L. resources, and to find the endophyte producing G. biloba flavonoids. [Method] With G. biloba leaves and roots as the plant materials, tissue block isolation method and streaking cultivation method were adopted for the Isolation and purification of endophytes. Withering solution of G. biloba flavonoids as the reference, TLC analysis on the fermentation liquids was performed. Al (NO 3) 3-NaNO 2 was used as the specific chromogenic agent of flavonoids. of eight kinds of endophytes were screened. LC analysis showed that the color band or spot of 07-Y8 fermentation liquid had the same color and similar mobility as the standard solution solution of G. biloba flavonoids. [Conclusion] Strain 07-Y8 might have the capability of being fermented into G. biloba flavonoids or other similar compounds, which provided new approach for the development and utilization of G. biloba flavonoids.
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