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目的 :了解B细胞淋巴瘤白血病 (LL)患者TCRVβ亚家族T细胞的分布及其克隆性。方法 :利用RT -PCR分别扩增 3例LL患者外周血单个核细胞的TCRVβ 2 4个亚家族基因的CDR3,以了解患者各Vβ亚家族的利用情况 ,然后将阳性的PCR产物进一步经荧光素标记和基因扫描分析产物的CDR3长度 ,了解T细胞克隆性。结果 :患者中仅存在 2～ 6个Vβ亚家族T细胞。基因扫描分析显示 ,2例患者外周血中的Vβ1、Vβ3或Vβ4亚家族出现克隆性增殖T细胞。结论 :LL患者外周血存在倾斜性分布和克隆性增殖的Vβ亚家族T细胞 ,这可能是机体T细胞受白血病细胞相关抗原的刺激作用而引起机体产生相应的特异性抗白血病的免疫反应。
Objective: To investigate the distribution and clonality of TCRVβ subfamily T cells in patients with B-cell lymphoma leukemia (LL). Methods: The CDR3 of TCRVβ2 4 subfamily of peripheral blood mononuclear cells in 3 cases of LL patients were amplified by RT-PCR to understand the utilization of each Vβ subfamily of patients. The positive PCR products were further transfected with fluorescein The CDR3 length of the product was analyzed by labeling and gene scanning to understand T cell clonality. Results: There were only 2 to 6 Vβ subfamily T cells in the patient. Gene scan analysis showed that clonal proliferating T cells appeared in the Vβ1, Vβ3 or Vβ4 subfamilies in the peripheral blood of 2 patients. CONCLUSIONS: There is a skewed distribution and clonal proliferation of Vβ subfamily T cells in the peripheral blood of patients with LL, which may be caused by the stimulation of leukemia cell-associated antigens by the body’s T cells and the corresponding specific anti-leukemia immune response.