本文对比了在离体条件下美洲大蠊消化道组织匀浆羧酸酯酶与胆碱酯酶对敌百虫的亲和力,前者比后者大23倍。模拟抗性昆虫羧酸酯酶活力增加的情况,将纯化的羧酸酯酶加入该组织匀浆中,发现胆碱酯酶结合敌百虫的能力降低,降低程度与加入纯化酶的量成线性关系。 This article compared the affinity of carboxylesterase and cholinesterase to trichlorfon in the digestive tract tissues of American cockroach under ex vivo conditions, the former 23 times larger than the latter. Simulating the increase of carboxylesterase activity of resistant insects, the purified carboxylesterase was added to the homogenate of the tissue, and the ability of cholinesterase to bind to trichlorfon was found to decrease linearly with the amount of purified enzyme added relationship.