利用甲基氚化胸腺嘧啶核苷(~3H-dT)与~(51)Cr释放法平行对比,检测正常小鼠脾细胞对YAC-1细胞的抑瘤率和杀伤率,两者呈显著相关。参与抑瘤效应的细胞与自然杀伤细胞(NK细胞)类似,都能被多聚肌苷酸:胞苷酸(polyⅠ:C)、白细胞介素2(IL-2)活化;耐受20Gyγ射线。实验表明,在抑瘤效应中,包含着NK细胞的作用。但~3H-dT掺入试验比~(51)Cr释放法更敏感,当选用低杀伤率的效靶配组时,仍能检测一定程度的抑瘤率。 Using methyl thymidine (~3H-dT) and ~(51)Cr release method in parallel, the tumor inhibition rate and killing rate of YAC-1 cells in spleen cells of normal mice were detected and both were significantly correlated. . The cells involved in tumor-inhibitory effects are similar to natural killer cells (NK cells) and can be activated by polyinosinic acid (polyI:C) and interleukin 2 (IL-2); they are resistant to 20Gy gamma rays. Experiments have shown that the effects of NK cells are involved in the antitumor effect. However, the ~3H-dT incorporation test is more sensitive than the ~(51)Cr release method. When a low killing effect target group is selected, a certain degree of tumor inhibition rate can still be detected.