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目的 初步探讨β 纤维蛋白原基因启动子区 4 5 5A/G多态性和血浆纤维蛋白原水平的关系以及在缺血性脑血管病中的意义。方法 91例脑梗死患者 (6 3 5± 10 1岁 )、74例无血栓老年对照组 (6 0 6± 10 8岁 )和 98例年轻对照组 (健康献血员 ) (37 5± 13 3岁 )。PCR RFLP (HaeⅢ )法分析 β 纤维蛋白原基因启动子区 4 5 5A/G多态性 ;血浆纤维蛋白原水平测定使用PT时间法。计量资料间比较使用t检验 ,由于纤维蛋白原浓度呈非正态分布 ,故检验前作对数转化 ;计数资料使用卡方检验。结果 H2等位基因频率在男性脑梗死组明显较老年对照组高 (2 2 7%和 7 1%,χ2 =5 5 6 ,P <0 0 2 ) ,在女性组中无统计学差异 ;在无血栓的所有人群中 (包括老年对照组和年轻对照组 ) ,H2等位基因频率随年龄增长的分布频率是 :≤ 4 0岁 ,2 1 3%;4 1- 5 9岁 ,15 4 %;≥ 6 0岁 ,10 2 %;男性老年和年轻对照组中 ,H1H1基因型人群血浆纤维蛋白原水平 (2 87± 96和 2 34± 5 8mg/dl)明显较H1H2 H2H2型 (331± 4 4和 30 7± 5 5mg/dl;t =2 5 3和 9 6 7,P <0 0 5 )低。在女性对照组中尚未发现同样现象。结论 男性人群血浆纤维蛋白原水平受β 纤维蛋白原基因启动子区 4 5 5A/G多态性的影响 ,H2等位基因可能?
Objective To investigate the relationship between 45 5A / G polymorphism of β-fibrinogen gene promoter and plasma fibrinogen level and its significance in ischemic cerebrovascular disease. Methods Ninety-one patients with cerebral infarction (655-110 years old), 74 thrombosis-free elderly controls (606 ± 10 8 years) and 98 young controls (healthy blood donors) (37 5 ± 13 3 years old) ). The PCR-RFLP (HaeⅢ) method was used to analyze 45 5A / G polymorphism of β-fibrinogen gene promoter region. The plasma fibrinogen level was determined by PT time method. Comparison of measurement data using t test, due to the non-normal distribution of fibrinogen concentration, so before the test for logarithmic transformation; count data using the chi-square test. Results The frequency of H2 allele in the cerebral infarction group was significantly higher than that in the elderly control group (22.7% vs 71%, χ2 = 556, P <0 02), but not statistically significant in the female group In all thrombosis-free groups, including the elderly control group and the young control group, the frequency of the H2 allele frequency with age was ≤ 40 years old, 21.3%; 4 1-59 years old; 15.4% ; ≥60 years old, 10 2%. The level of plasma fibrinogen in H1H1 genotypes in aged male and young control group was significantly higher than that of H1H2 H2H2 type (331 ± 4) (2 87 ± 96 and 2 34 ± 58 mg / dl) 4 and 30 7 ± 5 5 mg / dl; t = 2 5 3 and 9 6 7, P 0 05). The same phenomenon has not been found in the female control group. Conclusion The plasma levels of fibrinogen in male population are affected by 45 5A / G polymorphism in the promoter region of β-fibrinogen gene, and may the H2 allele be?