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核心样品种质库是体现作物品种资源遗传多样性并促进有效利用的一项重要资源管理机制。构建贵州稻种资源核心样品种质库时,须遵循既要保证最大可能的遗传多样性,又要避免过多的遗传同一性材料;既便于管理,更有利于利用和交流;既是数量上的精炼,又是特色性优异种质研究成果的集中体现等基本原则。采取形态特征与生物学特性鉴定为主,辅之同工酶等生化分析作为性状评价指标,分层取样,逐层聚类分析的方法,提出了贵州稻种核心样品构建程序按初级(30%)→次级(20%)→核心样品(10%)三个层次逐步开展。
The core sample genebank is an important resource management mechanism that reflects the genetic diversity of crop resources and promotes their effective use. To construct the Guizhou germplasm pool of core resources of rice germplasm resources, it is necessary to follow both to ensure the maximum possible genetic diversity, but also to avoid excessive genetic identity materials; both for management, more conducive to the use and exchange; both quantitative Refining, but also the characteristics of germplasm research results focused on the basic principles. Based on the biochemical analysis of isozyme and other biochemical characteristics as trait evaluation index, stratified sampling and layer-by-layer clustering analysis, the method of constructing Guizhou core samples based on primary (30% ) → secondary (20%) → core sample (10%) three levels gradually.