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[目的]建立酶联免疫吸附法(Enzyme-linked immunosorbent assay,ELISA)检测大鼠脑组织中舒芬太尼浓度。[方法]按舒芬太尼试剂盒说明,测定大鼠脑组织中舒芬太尼浓度,并考察日内与日间变异系数、回收率、精密度及稳定性。[结果]标准曲线舒芬太尼浓度0.0625～2μg/L时,线性良好,R2=0.9937,检测限为0.06μg/L。回收率范围(81.8±11.9)%～(86.9±7.1)%,日内与日间变异系数范围分别为(5.0～6.6)%和(4.8～9.6)%,稳定性好。[结论]用ELISA方法测定大鼠脑组织中舒芬太尼浓度,具有灵敏度较高,简便、快捷的优点。
[Objective] To establish a method for the determination of sufentanil in rat brain by Enzyme-linked immunosorbent assay (ELISA). [Method] The sufentanil concentration in rat brain tissue was determined according to the instruction of sufentanil kit, and the intra-day and inter-day variation coefficient, recovery, precision and stability were investigated. [Result] The standard curve of sufentanil concentration was 0.0625 ~ 2μg / L, the linearity was good, R2 = 0.9937, the detection limit was 0.06μg / L. The recoveries ranged from (81.8 ± 11.9)% to (86.9 ± 7.1)%. The coefficients of variation between day and day were 5.0-6.6% and 4.8-9.6%, respectively. The stability was good. [Conclusion] The determination of sufentanil concentration in rat brain tissue by ELISA has the advantages of high sensitivity, simplicity and speed.