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The generation of induced tissue-specifc stem cells has been hampered by the lack of well-established methods for the maintenance of pure tissue-specifc stem cells like the ones we have for embryonic stem(ES)cell cultures.Using a cocktail of cytokines and small molecules,we demonstrate that primitive neural stem(NS)cells derived from mouse ES cells and rat embryos can be maintained.Furthermore,using the same set of cytokines and small molecules,we show that induced NS(iNS)cells can be generated from rat fbroblasts by forced expression of the transcriptional factors Oct4,Sox2 and c-Myc.The generation and long-term maintenance of iNS cells could have wide and momentous implications.
The generation of induced tissue-specifc stem cells has been hampered by the lack of well-established methods for the maintenance of pure tissue-specifc stem cells like the ones we have for embryonic stem (ES) cell cultures. Using a cocktail of cytokines and small molecules, we demonstrate that primitive neural stem (NS) cells derived from mouse ES cells and rat embryos can be maintained. Future ed., using the same set of cytokines and small molecules, we show that induced NS (iNS) cells can be generated from rat fbroblasts by forced expression of the transcriptional factors Oct4, Sox2 and c-Myc. The generation and long-term maintenance of iNS cells could have wide and momentous implications.