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目的研究褪黑素对大鼠可卡因条件性位置偏爱复燃的作用及机制。方法建立大鼠可卡因诱导的条件性位置偏爱模型,在可卡因戒断后/复燃前给予褪黑素,检测实验大鼠条件性位置偏爱的变化,并应用Western blot和共聚焦激光扫描显微镜技术观察褪黑素对△FosB表达的影响;另外进行松果体摘除术,观察去除内源性褪黑素对可卡因诱导的条件性位置偏爱和△FosB表达的影响。结果褪黑素对大鼠条件性位置偏爱效应的复燃具有抑制作用,褪黑素下调了可卡因复燃诱导的△FosB在相关脑区的高表达。松果体摘除抑制了可卡因诱导的条件性位置偏爱的形成,但是对复燃诱导的条件性位置偏爱的强化无明显作用,松果体摘除组大鼠除了在海马表现出△FosB的低表达外,在其它脑区未观察到明显变化。结论褪黑素可能通过抑制可卡因复燃诱导的△FosB的高表达拮抗大鼠可卡因奖赏效应的强化。
Objective To investigate the effect and mechanism of melatonin on cocaine-conditioned place preference in rats. Methods A cocaine-induced conditioned place preference model was established in rats. Melatonin was administered before cocaine withdrawal / re-ignition, and the conditioned place preference of experimental rats was detected. Western blot and confocal laser scanning microscopy Melatonin △ FosB expression; additional pineal extirpation, observed removal of endogenous melatonin on cocaine-induced conditioned place preference and △ FosB expression. Results Melatonin had an inhibitory effect on the rekindling effect of conditioned place preference in rats. Melatonin down-regulated the high expression of △ FosB induced by cocaine in related brain regions. Pineal ablation inhibited the formation of cocaine-induced conditioned place preference, but had no effect on the re-ignition-induced enhancement of conditioned place preference. In addition to the low expression of △ FosB in the hippocampus, , No significant change was observed in other brain regions. Conclusion Melatonin may antagonize the potentiation of cocaine reward effect in rats by inhibiting the high expression of △ FosB induced by cocaine resuscitation.